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Pee tubes, Fungus gardens, and Weird frogs

Today we started a project exploring nutrient limitations in the forest canopy and on the forest floor. We are investigating this by peeing in tubes and strapping them to different trees (I promise there is real science behind this). We are going back out on the trails tomorrow morning to collect the pee tubes and count the number of arthropods we find in them.

While we were walking the trail to place our pee traps, Phoenix spotted a jumping pit viper in the middle of the trail and then Rusty spotted an eyelash viper on a log off the trail. The eyelash viper was especially exciting, because Dr. Solomon said he had never seen one in his twelve years of visiting Chiquibul. Later on down the trail, Ava spotted what they described as a “Spiky moth” on the trunk of a tree, but when they said it was dead, I suggested that it was actually infected with the Ophiocordyceps fungus, which turns arthropods into ‘zombies.’ Almost immediately after that, I spotted another, more decomposed Ophiocordyceps moth on a leaf on the forest floor. I still really want to see an ant infected with Cordyceps, but the moth was still really cool.

Moth infected with Ophiocordyceps

After lunch we searched nearby the research station for some leafcutter ant (Atta cephalotes) nests of differing ages to dig up and compare. First, we found a young (~1 year) ant nest with a single entrance ‘chimney’ or ‘turret’, and we started digging about a foot away to try to break into the underground fungus chambers. Dr. Solomon told us that the very young nests do not have big soldier ants for defense and that the individual worker ants are shinier than the ants in older colonies. After a few rounds of everyone taking turns digging into the nest, we finally broke into the fungus chamber, which looked like a whitish gray spongey honeycomb. Dr. Solomon started scooping out the fungus to look for the queen, and when we finally found her, I got to hold her and have my picture taken with her :’) When we tried digging up a slightly older nest, we kept digging and digging, but no ants were coming out, which made us think that the nest was abandoned. When I got my turn with the shovel, I dug down super deep and pried up a huge chunk of soil, and I exposed a Mexican Burrowing Toad (which we had learned about literally just yesterday)! We gently picked it up and it was SO gelatinous, we couldn’t believe that it could burrow into the hard packed clayey soils, but it had strong hind legs that it uses to burrow backwards!

My picture with the Queen!

After dinner, we went on an optional night hike to the frog pond, which was unforgettable. At the frog pond, we saw not one, but TWO endangered Morelet’s Tree Frogs. We got quite a few good pics and vids, and they even jumped and crawled on a few of us. One jumped onto my hand!!! A critically endangered species reached out and touched me! We also saw not one, two, three or four, but FIVE arboreal snakes in the branches and canopy above the frog pond hunting for the gelatinous globs of tree frog eggs plastered onto the underside of leaves. At least one was successful in getting a mouthful of frog eggs, but hopefully there are still enough for a new generation of frogs.

Tree frog eggs!
The critically endangered Morelet’s Tree Frog

Totally psyched that we hiked

Today was our first (and hardest) full day of hiking in the rainforest! We started with an early-morning birding session fueled by coffee and awe. We heard melodious blackbirds, and saw black vultures, turkey vultures, ocellated turkeys, plumbeous kites, TWO toucans, and a collared aricari.

After a hearty breakfast, we drafted an experimental design to characterize the habitat use of predators and prey in the Chiquibul, and we suited up for a long hike to set up some camera traps.

Into the jungle (Pre-first hike)
Out of the jungle (Post-first hike)

Dr. Solomon gave me an aspirator to suck up ants into a little viewing chamber for easier identification. I didn’t get to (or need to) use it today on our hike, but it is very cool, in theory, to be like an anteater slurping up ants in the rainforest. I did see a new ant species today: the army ant (Eciton sp.)! At first, I thought it was another leafcutter (Atta cephalotes)  trail, because they were moving in a straight line rather than the carpet-like swarms that are associated with the army ant species that I know of (E. burchellii), but their conspicuous reddish-color and large soldier class still identifies them as army ants.

I also saw the same type of Ponerine/Hypoponerine ant that I saw at Caracol yesterday, and this time I got some really good close up pictures, so hopefully I can consult the ant guide book and get a positive ID.

We also saw an absolutely humungous leafcutter ant nest deep in the rainforest on our hike (we set up a camera trap there, so we have an excuse to go back in a couple days). The mound was probably four feet tall and several meters in radius with dozens of entrance tunnels and ventilation shafts! Dr. Solomon said that the entire biomass of the colony was about equivalent to that of a cow, and that they probably consume about the same amount of vegetation per day.

A superhighway of leafcutters

Caracol and Chiquibul

Today was chalk-full of adventure, history, and changes. We woke up early at TEC and made three field-ID’s before breakfast! First, I spotted a Shimmering Golden Sugar Ant (Camponotus sericiventris) and I told everyone that would listen to me about golden ant butts. Then, I let McKenna borrow my binoculars and she identified both a Great Kiskadee and a Social Flycatcher flitting about high up in a pine tree. Lastly, on our way into breakfast at the dining hall, we spotted an agouti (which promptly ran away from us).

We left TEC after breakfast and were on our way to Caracol Archaeological Reserve and then to Chiquibul National Forest.

Caracol was absolutely stunning. I have never visited any ancient ruins, and it was incredible to learn about the Mayan civilization and culture. The site of Caracol is vastly underappreciated  and under-documented. Archaeologists have excavated several huge temples and astronomical buildings, but the entire complex is a seven mile radius with more than 35,000 individual structures (at its peak). I think the most fascinating building at the site was the ‘E-group’ astronomical building, whose three peaks align with the position of the rising sun at three key points throughout the year! As I climbed up the opposite building to see what the ancient Mayan astronomers did, it started to pour down rain (our first taste of Belize’s wet season), which made the uneven mossy steps even slipperier.

Slippery stone steps

We saw some very large leafcutter ant (Atta cephalotes) colonies (more than a meter in diameter) and also a very small one! Dr. Solomon said that the small one was probably a year or two old, because it only had two entrance holes to the nest.  I also saw a very large ant with mottled/spotted coloration that I didn’t recognize. I asked Dr. Solomon and he theorized that it could be a Ponerine or Hyperponerine ant.

Ponerine (?) ant

Forest day 3: wANT to look at Ants? (06/18/22)

It’s already our second Saturday on this trip. We get back on Wednesday. Time is passing so fast. This morning for breakfast they changed it up with some ham and bread with the beans and eggs rather than bacon and tortillas. It wasn’t a coffee morning for me though.

After breakfast, Michael presented his lecture in tropical soils, during which we were all told to hydrate and drink our water. After the lecture, we did an experimental design for testing the abundance of arthropods on the forest floor vs the canopy, in nutrient rich conditions (pee tubes) vs nutrient poor conditions (water tubes). After planning, we then went to pee into tubes. The setup for this experiment is: at breast height on a tree, one water tube and one pee tube will be wired onto opposite sides of the tree, and then at the base of the tree, with the same orientation, the second water and pee tubes will be put flush into holes in the ground.

The horrific vials *shudder*

The tubes are going to be picked up tomorrow for collection and counting of arthropods. I didn’t actually go out with the group to put the tubes up since my ankle was hurting from twisting it yesterday, but my tubes were placed in the field by someone. While they were out, they apparently ran into a jumping viper (venomous) and an eyelash viper (also venoumous) that someone who has worked here for 12 years hadn’t even seen.

Eyelash Viper (Bothriechis schlegelii)
Tiny jumping viper (Atropoides picadoi)

The group returned at lunch, which is when I rejoined them. After lunch, we went exploring around the field station clearing on the search for small leaf cutter ant nests. Once we decided on one, Scott instructed us on how to dig next to it to find the fungus cavity. This nest was young, only about a year old, so it was quite small. We saw some seargent majors (large worker ants), but no soldiers (large defense ants) since the colony was so short. He dug out some of the fungus from in the cavity, and eventually pulled out the queen for viewing. She was huge!

In an attempt to excavate a larger colony (which ended up being abandoned), we found a Mexican burrowing toad!! They are super funny looking guys, with gelatinous bodies, splayed legs and almost no neck. I didn’t get to hold him since I was wearing bug spray, but he was such a rare and cool find. 

Mexican Burrowing Toad

Scott then excavated another colony, solely to show us what soldiers looked like. Some people picked them up, and even let them bite their shirts?? It’s a no from me dawg. 

 

I didn’t have much exposure to new trees today, but while we were in the clearing looking at the ant nests, we noticed some almost blackberry looking things on the ground, which I identified as some sort of cone, that were very light and had a woody consistency when cut into. These cones are the seeds of the bay cedar (Guazuma ulmifolia). I also believe I saw a small Billy Webb tree (Acosmium panamensis) along the edge of the clearing based on the shape and color of its fruits, although I could be wrong. 

We finished up the day with the lectures of the day before dinner, with free time or an optional night hike (which I did not choose to go on, again, because of my ankle).

today is also the day that I learned that I am covered in chigger bites on my arms from yesterday’s falls. They’re so itchy, I hate it.

That concludes day 11 – can’t believe we’re so far in. 




Into the Rainforest: Cool Views and Camera Traps

Today we had our first introductions to the Chiquibul rainforest! We got to come up with a new research project using a bunch of camera traps to get pictures of animals that we might be scared away if we tried to see them in person. We set up 16 total camera traps all around the research station to see which predators and prey of the forest use areas of human influence such as trails and roads differently. This gave us a great opportunity to spend pretty much all day hiking around the rainforest and getting to know the area! 

On these excursions we saw so many cool things! We could look up and see so many different trees, palms, and other epiphytes such as bromeliads. Dr. Solomon also showed us a truly magnificent leafcutter ant nest that was nearly 20 years old and ridiculously huge! At the peak of one of the trails, we climbed up a tower that looks over the whole rainforest with nothing but trees for miles and miles! And then on the way down, we found a small cave that the Maya used, which was so beautiful and we even found a small area of ceramic sherds! 

Within this cave, and throughout the entire hike, I found so many examples of cool arachnids! Along the trails in the leaf litter, I saw (and held!) some harvestmen, which are a class of arachnids that include daddy long legs. I also saw so many different types of webs, such as thick funnels in the tree trunks. Some of the coolest ones we saw though, were the huge black spiders hanging from the ceiling in the cave! Although I couldn’t identify these sentinels (or get good pics because my camera died), it was still awesome to see so many of them! 

Spider egg sac found along the trail

Our Fastest Hike!

Hey guys! It’s Michiel again : )

The class was up early to collect the pitfall traps we set yesterday. We felt like real TFBs as we were out there because we went out there without a professor to lead us. We were able to pick up all the traps in about 30 minutes. We went back to the station with our tubes, poured out the contents in a petri dish, and picked out arthropods with forceps. We ended up collected five arthropods from the canopy traps filled with water, seven arthropods from the canopy traps fill with pee, twenty arthropods from the floor traps filled with water, and twenty-six arthropods from the floor traps filled with pee. We were able to conclude that there are more total bugs on the forest floor than the canopy, and we believe this is true because the forest floor is more nutrient rich than the canopy due to falling organic matter. We also saw that, in general, the arthropods preferred the pitfall trap with nutrients; however, the data shows that the arthropods in the forest floor may prefer it more than the ones in the canopy. We believe that maybe arthropods in the canopy have adapted to less available nutrients and thus don’t seek them out much, unlike the ones on the forest floor, but we’re not too sure about that reasoning.

After analyzing our data, Adrienne, Ava, Sophia, Elena, Rusty, Michael, McKenna, and I went out to collect the camera traps we had set up the other day. We went through the trails really fast, sweating through our clothing at some points. Eventually, the rain started, which cooled us down, but we were able to get through the trails that took us the entire day on Friday in only an hour and a half. After collecting the traps, we went back to Las Cuevas where we noticed the incessant sound of Mexican Burrowing Toads coming from Frog Pond. A couple of went out to go look at them, but, unfortunately, they all seemed to be hidden in the thicket that surrounded the pond. However, it was really cool to hear the croaks coming from all directions. Here’s a picture of us there!

The rest of the day was fairly uneventful. However, we did get to see a dog on the road! I finally saw a mammal, you guys. My phone was dying, though, so I couldn’t take a picture. I’m sure you all know what a dog looks like. It was brown and white and the breed of dog was indiscernible.

Hopefully, I’ll get to see some wild mammals soon!

Completely Nerding Out: A Great Day for Archeology and Arachnids

Today we left the lovely TEC to journey into the Chiquibul rainforest! We had a long and bumpy car ride through the capital city Belmopan through the Maya Mountains, to our first stop: the Caracol Archeological Reserve! As someone who is also majoring in anthropology/archeology, this visit was so insanely cool! We saw multiple different plazas, ballcourts, and complexes, and got to walk up and explore one of the large stone structures, the top of which is the highest manmade point in Belize! The view was so vast and impressive! We also saw these enormous ceiba trees which had giant buttresses and intertwining roots

I also saw the first signs of my rainforest taxa which are arachnids! While we were at the site, there were a few dark and moist recesses in the stone walls, where we just barely spotted a tailless whip scorpion ! These dudes look pretty freaky, but they are non venomous and won’t hurt you. They usually like living in caves, so I wasn’t expecting to see any until our last day spelunking adventure! 

 

After this visit, we made another long car ride to Las Cuevas Research Station. We had a short orientation to the site, but spent most of our time last night doing lectures so we haven’t really gotten to explore yet. That’s for tomorrow’s adventure!

Our Luckiest Day!

Hey guys. It’s Michiel again : )

Today was really eventful. It started off slow with breakfast and a lecture in the morning, but it quickly picked up. At the end of the lecture, Scott had a discussion with us on nutrient availability in the canopy and the forest floor, and he related it to arthropods. Together, we came up with the question “How does nutrient availability influence arthropod abundance in the canopy and forest floor?” After we came up with this question, Scott told us that the way we’d be using nutrients for our experiment would be by peeing in tubes and using those tubes as traps. This was really surprising to all of us, and we promptly drank as much of our water bottles as we could. After we filled our tubes, we went out to the Mayan Trail to set up these pitfall traps. Basically, we each put one tube of pee flush against the trunk of a tree and another tube of water on the opposite side of the trunk. We also put a tube of pee and a tube of water in the soil at the base of the tree. Arthropods are expected to fall into these tubes.

While we were going through the path to set up these traps, we passed by a tree with scratch marks and an indentation in the ground at the base. We believe it may have been one of the cat species using the tree to sharpen/shorten its claws, but the scratch marks seemed to high and the indentation too big for it to be a margay (Leopardus wiedii) or an ocelot (Leopardus pardalis). However, they were too low and it was too small for it to be a jaguar (Panthera onca) or a puma (Puma concolor). My best guess is that it may have been a juvenile jaguar or puma.

Tree with scrapes
Indentation in ground

After a couple of hours, we finished setting up our traps and went back to Las Cuevas for lunch, After lunch, Scott and Adrienne sent us out to look for some leaf cutter ant nests in the clearing that surrounds the station. Elena and I didn’t find any, but we did hear something in the brush that kind of sounded like a monkey. We know that it wasn’t a black howler monkey (Aloutta pigra) because it sounded nothing like a black howler monkey, so we think it may have been a spider monkey (Ateles geoffroyi), but we couldn’t see through the brush, so we have no idea. After we heard it, though, someone called out that they had found a leaf cutter ant nest, so we went over there to check it out. Scott told us that it couldn’t be more than a year old, which is exactly what we were looking for. We began to dig right next to it so we could see the fungus garden. After a while of digging, major ants came out to defend their home. Ava and Elena kept trying to pick it up, and eventually they did! I’m proud of them. After a while of digging, we saw the fungus garden, which is gray, and then Scott pulled out the queen, which we each held. After achieving our goal, we went to a new nest that was older. However, after a while of digging, we couldn’t find anything ­– there weren’t even any ants. Before we gave up, though, we saw a Mexican Burrow Toad! We thought that it would be impossible for us to find this frog, but we all really wanted to because it’s so ugly (but in a cute way). I’m really glad we found it. Anyway, we went to a new leaf cutter nest after we put the toad back. This nest was much larger than the last, and it had soldier ants, which are much larger and bite harder than the major ants. After a while, we were able to get to the fungus garden and Elena and Ava picked up the soldier ants!

Mexican Burrowing Toad

After the leaf cutter ant nests, we had lectures, then we had dinner. After dinner, we went on a night hike! The night hike was crazy. We saw two Morlet’s Tree Frogs, which are endangered, and we say nine snakes. It was really cool seeing all the snakes because we would think that we had found them all and then someone would find a new one. We were fixated on one of the snakes for 20 minutes, which we saw eat some red eyed tree frog eggs, which are also endangered. After we watched the snake eat those eggs, we went back to Las Cuevas, worked on our blogs and field notebooks, and went to sleep.

I’m really hoping I see more mammal-related stuff tomorrow, but I know for sure that I’ll keep you guys updated!

First Day of Hiking!

Hey y’all! It’s Michiel again : )

I woke up today at 5:45 for my first full day at Las Cuevas. I was planning to wake up at 6:30, but the birds were so loud that I couldn’t stay asleep. Also, last night there was a black howler monkey (Aloutta pigra) right next to the station that was yelling at another black howler monkey elsewhere in the forest. It definitely kept me awake longer than I wanted to be. Anyway, I joined my fellow TFBs on the patio around 6 and we birdwatched together. I saw three beautiful turkeys, and a couple vultures. Later, we had breakfast, then we were tasked to come up with a research question that involved using camera traps. We came up with “Where are different functional groups (large/small predators and large/small prey) most abundant (forest, trail road)?” To answer this question, we decided to set up camera traps throughout forested areas, on trails, and on roads.

We left the station around 10 to begin setting our traps. For the first two hours, we were all so amazed by the surrounding fauna and flora that we only managed to set six traps – one on a trail and five on a road. While we were in the forest, we actually saw Scarlet Macaws! I’ve included a picture of them here.

After setting these traps, we made our way back to the research station for lunch at 12:15. Lunch was kept pretty short because we needed to go back out there and set more traps. When we went back out there around 1, we traversed through a trail with much more hills (it was actually almost completely uphill then completely downhill) than we had experienced on the other trail. We stayed on this trail until about 6:15 and managed to set 4 traps on the trail, 5 traps within the forest, and 1 trap near Frog Pond. While we were hiking this trail, we saw some beautiful bugs that Caio kept picking up, a cave with ancient Mayan pottery in it, and a lot of leaf cutter ants. We also came across a termite nest, and Scott told us to take termites from it and eat them, which, of course, we did. They tasted really fresh – like carrots. We also went up the bird tower, which had some amazing views.

Unfortunately, I did not get to see any mammals. However, we did hear a black howler monkey yelling while we were placing road traps in the morning. I’m expecting that any sightings of mammals will be really rare throughout the next couple of days, but hopefully the camera traps will take a lot of good pictures of them.

The rest of our day went pretty smoothly. We had dinner, had a break to recuperate from the exhaustion we were experiencing, then had some lectures. I’m hopeful that tomorrow I’ll get to see some mammals!

Journey to Caracol and Las Cuevas!

Hey guys! Once again, it’s Michiel : )

We woke up this morning at the TEC (one of the most beautiful places ever) and quickly got ready for breakfast at 6:30. After breakfast, everyone gathered their luggage near the side of the road and got into vans for our journey to Caracol. On our way to Caracol, I got to sit in the front seat, where I prepared for my topic lecture that would be happening later that evening (spoiler alert). I read a bunch of papers with tiny letters while we were traveling on very bumpy roads. I may have gotten a little carsick, but it was only a little headache, and I was committed enough to presenting a wonderful lecture that a headache wouldn’t stop me. I managed to read everything I needed to by the time we got to Caracol. There, we saw a HUGE Ceiba tree, Mayan middle-class homes, and a beautiful pyramidal temple. We climbed to the top of that temple, looking in ancient bedrooms and tombs on the way up. Once we finally got to the top, we got a truly amazing view of the Chiquibul Forest. While we were there, we also saw white lined sac bat. After the pyramidal temple, we saw their place of astronomical observation, an area where they played a ballgame, and an aquifer. After all this, we had lunch, during which we were able to hear Black Howler Monkeys (Alouatta pigra) screaming throughout the forest. I, unfortunately, wasn’t able to get any pictures of these monkeys, but here is a picture of us on top of the temple and me next to a Ceiba tree!

After lunch, we left Caracol and continued our journey to Las Cuevas Research Station. On our trip, we saw three turtles crossing the road, two scarlet macaws, and a toucan! Once we finally reached the station (around 4:30), we were shown around. The station is really nice; I think I’m going to really enjoy it here. After our orientation, we began catching up on lectures that we had fallen behind on. After two taxon lectures, we had dinner, then I presented! I presented on life in the rainforest canopy, and I think I did really well, but we’ll see. After I presented, there were a couple more lectures, then we worked on our blogs and field notebooks and went to bed.

I’m really excited for everything to come. I’ll be sure to keep y’all updated!