Tag Archives: penicillus

Day 3: Penicillus Project + Coral Graveyard

Today we started to think more like field biologists by using tools such as our quadrates and transect tapes to measure densities and volumes of objects such as coral heads! We came up with a scientific question and hypothesis to test about the green algae, penicillus. We wanted to test how the penicillus density would change as we got into deeper waters. Our class could perform this data collection if we all went vertical by shoring using our transect to measure our 100 ft from shore, and then using our quadrat to search for and identify penicillus among the sea grass. Our hypothesis was that penicillus would be more abundant in shallower waters, due to higher sunlight and more nutrients, but we were wrong. Our data supported the idea that penicillus was more abundent in deeper waters and was typically seen not solitary but in groups. We presented our evidence and conclusions to our professors and they seemed impressed for our first field biology project. Maybe some day in the future we’ll try to experimentally determine the reason for this (possibly competition with sea grass or other factors).

Here’s us working and discussing our presentation of our data

While we were working on our presentations Nyala and Caio brought us coconut meat which was a delicious snack!

Later we visited our Professor Correa’s favorite place on earth- the Coral Graveyard. The coral graveyard has all different specimens of corals that seem to be very well preserved.  There was stag horn coral, different species of brain corals with cool patterns and ridges, and there was also a type of coral that is so rare that there has hardly been any sightings in the last 40 years (before the coral disease epidemic). It was very important that we know learn to match these corals up with live species that we may encounter in the reef! We also discovered a fossilized palm tree species which Dr. Solomon is pictured holding!

I spotted a coral specimen that had  a possible annelid boring mark. This was probably a type of worm hole! It was perfectly preserved and I wonder what type of annelid could have made that mark.

There was also some specimens of Bladed Fire Coral (millepora complanata) which is a common hydrozoan reef-building coral in Glover’s reef! Bladed fire coral has very small hair-like polyp holes compared to most other species I have encountered.

I can’t wait to see more of Glover’s tomorrow and hopefully go on a boat snorkel trip!

~Maegan

The Best Place on Earth (Day 3)

Hi all, it’s Faith with Day 3 updates from the 2022 Belize trip!!!

Today we started off with a new activity where we learned how to use the quadrats and transect tapes.  On the reefs, quadrats are used for making standardized measurements while fighting the wave currents. You can count them using  the individual squares or their cross-sections!!! We took on two tasks, one as a duo and one as a whole group, that challenged us to use quadrats and transect tapes to answer a scientific question.

As a pair, Maegan and I tried to measure the heights and widths of young palms (we called them coconut palms because they still grow out of coconuts) on the Glover’s island, but Dr. Correa told us to change it, so we ended up measuring the volumes of two random coconut palms. We used the transect tape as a tool to decrease the bias in our samples, and we used the qaudrats squares as a unit of measurement.

After this test-run, our entire group created a scientific question, hypothesis, and procedure for collecting data. Here are the details:

Our question was, “How does the density of the green algae Penicillium change with distance from the dock?” Our hypothesis was that the density decreased because “algae are light dependent and nutrient depended,” and we *assumed* that there was more light and nutrients towards the shore.  For our actual experiment, our pairs lined up horizontally at the doc, and then layed out 100ft of transect tape in a line straight ahead of us. Then, every 10 feet we counted the number of Penicillium in 1 quadrats range on either side of the transect tape. Our findings actually conflicted with our hypothesis because the distance with the highest Penicillium density was actually 80ft away! We concluded that our hypothesis may be wrong because 1) seagrass was outcompeteing the algae in shallower waters, 2) the waters by the shore might not be more nutrient rich or provide more light, and 3) we may have gotten better at finding the algae the more we practiced (therefore reporting more at deeper depths). However, we kinda ruled out #3 because Maegan and I did the experiment backwards, and even though we reported the algae from 100ft to 0 ft, our data aligned with the trend (we found 6 Penicillium at 80ft. which was our highest density). Another group also did a backwards collection and had similar data. To finish off this trial research, the professors made us present it just like I said it to you now!!! So, look below for pictures of our beautiful poster!

Then data collection dive was challenging in many ways. I found out how difficult data collection is because of the currents and carrying materials. Because of this, I ended up leaving my camera behind, so of course, the worst thing happened– 2 echinoderms showed up! First, Professor Correa brought me a Oreaster reticulatus more commonly known as a cushion sea star. I was not sure where she found him, but I assume it was in the sea grass where we were collecting data. I had a difficult time identifying this star because it had pillow-star depth, but the spines were the same color as the bodice and the legs lacked a prominent “fused” appearance. Most guides show pictures where their spines are lighter than the bodice color and their legs are very fused. Nevertheless, I got to hold him and feel his spiny tube feet prick my fingers. Because I didn’t have my camera, someone else took my photo, so hopefully I’ll be able to get the picture and upload it for the next blog!

Next, I saw a West Indian Sea Egg (Tripneustes ventricosus) just sitting in the sea grass, but Ruth, our marine safety guide, picked him up before I could. So, alas, I did not get to name him “Fluffy,” and I cannot cross this off of my goals list! Anyways, we got to see the interesting sea urchin suction mouths because he suctioned to us while we held him. Shortly after seeing him, messing around with plastic-bag jellyfish, and trying to grab upside-down jellyfish, we went inside for lunch.

For our last activity of the day, Dr. Correa brought us to “the best place on Earth” where we identified washed-up coral skeletons based on their coralite and polyp structures. It was a very informative talk, but I don’t think I can cover it all here. You’ll just have to visit the coral graveyard yourself with a coral guidebook! I did go a littttttllllleeeee conch crazy and collected every conch that had color in it (about 5 or so). I also found a walking stick!!!

Till tomorrow!

Quotes of the Day:

“This place is amazing!!! *points at coral* And this is Agaricia”

“Signing off in another language would be a very suburban mom thing to do, ‘like I learned a few new words today’ would be too ‘mom’ of me”

West Indian Sea Egg (sadly not named Fuzzy)
Tripneustes ventricosus
Our presentation poster with a quadrat heart <3
Labeling Coral from the coral graveyard
Fossilized Palm Roots from the coral graveyard. This one stumpted us at first; we knew it wasn’t a coral. All we could do was ask Prof. Solomon, “Is this a land thing?” We found out what it was by finding dead palm roots that looked very similar.

today powered by coconut

Today was a big day. I know you were all waiting in suspense after the cliff-hanger ending to yesterday’s blog post. Well, wait no more.

We started out the day by practicing using out quadrats (which are actually 2×2 feet-oops!). We had to come up with a scientific question that we could answer by using the quadrat. Sophia and I chose to ask what percentage of the sand is occupied by live foliage. We laid out 30 feet of transect tape, then placed the quadrat on either side every five feet, and counted the number of squares that contained a live plant. The answer we came up with was 22.7%.

The next step was to figure out a scientific question that we could answer with the quadrat in the reef, collect data, and present our findings to the professors. You can read all about it below!

Also, I must note that throughout our scientific process we were being supplied fresh coconut by the kids. #sponsored

After that, we went on a walk to the coral graveyard-Professor Correa’s favorite place in the world! It looked like a beach full of gray rocks, but upon further inspection, it turned out to be fossilized corals! We were able to identify the corals based on the shape of the calyxes (the little spot that the coral polyp inhabits). In live coral, the skeleton is covered by the tissue, so in these fossils, we could easily see the identifying markers. Some of the fossils belonged to corals that have been nearly wiped out by disease and are rarely seen in nature, so the coral graveyard was truly a special place.

I also saw some sargassum that had washed up on the shore of the coral graveyard. I think it was sargassum natans VIII, but it was hard to tell because most of it was dead. There were also some floating sargassum patches out at sea, which was cool because there aren’t any that I have seen within the lagoon.

We then came back to watch the presentations for the night. I delivered mine on nutrient pollution, overfishing, and the future of the coral reefs.

And that was it! Tomorrow we are looking to go further out via boat to see new reefs, weather allowing of course. Stay tuned!

-Elena

ps. happy graduation to my brother! (although I doubt he’s reading this)

Quadrats + Coral Graveyard

I felt like such a tropical field biologist today (with training wheels of course). We started off the day with a land activity using transects and the quadrats that we made yesterday. Liliana and I got some practice using the gear and with the methodology by gathering data to characterize what percentage of the measured area had evidence of hermit crab movement through the sand. Here is a photo! (The square is the quadrat, the line is the transect, and the orange rectangle is a very fancy dive clipboard)

Then, all of the TFBs put our heads together and came up with a question to test with this procedure out in the seagrass beds. We chose to investigate how the density of penicillus (a green algae) changes with the distance from the shore. We decided on a uniform methodology and got to work! I saw a lot of warty corallimorphs and great Caribbean sea anemones in the seagrass beds! After we collected our data in the water, we came back to the wet lab and compiled our data. We all made a poster that reflected the entire experiment, including results, discussion, and figures (one of which is a taped-on penicillus in a quadrat heart).

While working in the wet lab, I ate some freshly husked coconut as a snack!

Later in the day, we went to what Adrienne called the “coral graveyard,” and which she also described as “the most amazing place on the planet.” It really was amazing! The field of coral skeleton rubble was extremely well-preserved, and we were able to identify a lot of the species! One thing that I was particularly excited about was seeing and correctly identifying Montastraea cavernosa fragments. I took Adrienne’s coral reef ecosystems class, and my semester-long research proposal centered around this coral. It was so gratifying to get to hold a piece of its skeleton framework!

Besides the few sightings I got in during our penicillus data collection, I didn’t have a lot of time or opportunities to focus on my taxonomic group today. However, I am now so much better at recognizing great Caribbean anemones and warty corallimorphs!

Although my collection of mosquito bites has grown to be rather impressive, I can’t wait to get more if that means I can have more days like this!

– McKenna

Day 10: Quadrats and Transects

We started off this morning with a refreshing snorkel session at 8:30am. This time we brought out more gear, specifically a clipboard with waterproof paper to take notes of what we saw. There was a general feeling among all of us that the waterproof paper had to be some sort of black magic, but it worked so we stopped questioning it. Holding the clipboard and trying to take photos and write notes while avoiding being pushed into the coral by the currents took a long time to get used to, and I definitely haven’t mastered it.

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Gathering data in sea grass beds

To make everything more confusing, we added more gear after lunch. We performed a short experiment to practice using transect tapes and quadrats. The question that we were trying to answer was whether Halimeda or Penicillus species had a higher abundance and density in the sea grass off of the pier at Middle Caye. We worked in 6 groups to run transects across the beds of sea grass and counted the number of individuals of each species in quadrats along the transects.

In the end, we found 153 Halimeda individuals and only 1 Penicillus individual in the 216 square feet that we measured. We noticed that even slight differences in the composition of the sea grass bed, such as the density or length of the grass, changed the likelihood of algae being found in the plot. To quantify these differences we would have to run another experiment that focused on the different zones in the sea grass bed. Our experiment was limited because of the small area that we covered and difficulty that we had counting the individuals. Even though we were in water that was 8 feet deep at most, it might have been easier to run the experiment using SCUBA so we wouldn’t have to keep surfacing, which made counting confusing when we had to see under blades of grass.

Halimeda and Penicillus are interesting green algae because they are calcareous. Halimeda incorporates calcium carbonate into its thallus in flat chips, whereas Penicillus has calcium carbonate in the brush-like filaments at the top of the algae. The different forms of calcium carbonate lead to different types of sand. Halimeda creates large, flat grains of sand. The sand from Penicillus is finer and muddier.

Day 9: The Reef!

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View of Glovers Reef from observation tower on Middle Caye
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Patch reef in Glovers Reef

Today we traveled from the zoo to Glovers Reef. Glovers Reef is one of three atolls in Belize. It consists of four islands – Southwest Caye, Middle Caye, Long Caye, and Northeast Caye. We got to the island around 3:45pm and were able to snorkel for an hour before we had dinner. To our surprise, the water was like bathwater, especially right by the pier. It almost wasn’t refreshing, but being in the water was amazing.

Most of the sea floor that we saw was covered in sea grass. There weren’t any trees in the sea, but someone found a Penicillus capitatus, which is a species of green algae that looks like a paint brush. A little farther out, the sea grass gave way to a few patch reefs. The reefs had more sediment than I was expecting, but the diversity on the reefs was still greater than almost all reefs I have visited. The first species that I noticed were Gregonian sea fans, that were purple and rose gracefully from the mounds of coral.

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Halimeda sp.

The sedimentation on the patch reefs made them a good habitat for green algae. The highest concentrations of green algae that I found were along the edge of the patch reef. There were multiple species of Halimeda, but I wasn’t able to identify the exact species. There was a lot of Caulerpa cupressoides, which was smaller than I was expecting but still very recognizable. I also saw a species that could be Anadyomene stellata, but I need to look at the morphology of the algae more closely.

Tomorrow I’m looking forward to having more time in the water and learning about more reef species.

Day 10

Today was our first complete day on the reef. I can’t really put into words how lovely it is here. Sure I have sand on just about every surface of my body, but I am thoroughly enjoying my time here. The day was centered on two tools: the transect and quadrat.

First, we tried to quantify the percent cover of crab holes on the paths around the station (using the quadrats/transect on land). Honestly, my group did not get that much data (only one half of a square was covered over our entire transect).

This was then scaled up for our afternoon activity, transects searching for two geneses of green algae (Halimeda and Penicillus). We were trying to answer a similar question to the percent crab hole cover. We wanted to know if Halimeda or Penicillus would be more abundant in the sea grass bed.

Again, my groups struggled to find anything on our transect, with it being placed in some of the thickest grass. Either way, our data showed a significantly higher amount of Halimeda over Penicillus.

On the reef I continued to see Gorgonian sea fans, as well as whips and rods. I think that the future challenge that I will face is distinguishing between soft corals that are branching, yellow/brown, with them all being of similar morphology. Tomorrow on the more distant reef, I hope to see more.