Tag Archives: ants

Course Wrap Up: an “Un-Belize-able” experience

This class was nothing short of amazing. While it was taxing (so far from home, so much energy every day,) it was still an incredible experience. In my initial blog, I mentioned that I expected to be wowed- and that I most certainly was. One thing that wowed me: how cool ants and wrasses are! My research was cool, but seeing them and their behaviors first hand was truly something else. I loved seeing my taxa out in the wild! Another thing, my physical capability to live there. Las Cuevas and Glover’s are two fairly simple places to live – minimal internet connectivity, no AC, Clivus (mentioned in my glover’s day one blog,) just to name a few. Not only did I survive, I thrived.

On the topic of thriving, I’ll mention my favorite and least favorite parts. To get it out of the way, clivus was my least favorite part. He was character building, but I could have used a real flushing toilet. My favorite part, however, is hard to put into one single favorite part. So I’ll do two: one personal growth, and one TFB-related. Personal growth wise, this was the first time I have done something totally new and away from family in a while. I was sad and stressed those first couple of days. Once I realized what was happening, I was ecstatic. (Important context here: I am asleep away camp counselor) I realized that I was experiencing what my campers were. They feel this way at camp for the first time, and because my first time was so long ago, I couldn’t know how they feel. Huge personal and camp-professional growth totally unrelated to TFB. On the topic of TFB, my favorite part of the course must have been either excavating the Leafcutter nest or dissecting the lionfish. In these two cases, I got to see my taxa up close and see my research in action_ I researched fungal gardens of Leafcutters and lionfish invasive predation on wrasses, and seeing it IRL after all of that research was like nothing ever before.  The personal growth, leafcutter excavation, and dissecting a lionfish are three things I will certainly never forget. I will also never forget our visit to the ATM cave and all of the rich Maya history in the region- I would have loved to go back in time to see the cave being used IRL, but the remnants they left (except the human remains) were still so awesome and fun to see!

Another great part of the course was the ability to compare and contrast the two different ecosystems we visited- the reef and the rainforest. Right off the bat, you can guess they are fairly dissimilar- one is in the air, the other in the ocean, so how similar can they be? Well, the answer might surprise you. Because of the nature of the tropics (more sun in the days and more direct sun) You can find both the reef and the rainforest using it in cool ways. Rainforests have much richer canopies than floors or soils, and reefs are somewhat similar, they are also considered to be “nutrient poor” despite their rich biodiversity. Another favorite comparison of mine to make was connecting my taxa to the opposite ecosystem. I attempted to find the ants of the sea, which could be corals, glass shrimp, or even wrasses, while we determined birds were the wrasses of the sky (not the other way around, according to Dr. Evans.)

Overall, this course was so fantastic, and I highly recommend it for anyone in the future considering applying, you won’t regret it!

Images:

Boat Ride Away from Glover’s 🙁
Conch Shell on the Beach
Me + Other TFB’s enjoying a N&Y (nap and yap) session on the dock
Sunrise on the Bird Tower
Me, Dylan, and Lily on the bus to IAH from Rice on 5/16!

 

Final Blog: Reflecting on Belize!

The tropical rainforest and the coral reef are two of the most biologically diverse ecosystems on Earth, and I’ve come to appreciate how much they actually have in common despite their differences in location and appearance. Both ecosystems are incredibly productive due to consistent sunlight and warm temperatures year-round, which support high rates of photosynthesis—through dense vegetation in rainforests and through symbiotic algae in coral reefs. This productivity forms the foundation for complex food webs that can support a wide range of organisms.

What also stands out to me is the structural complexity in both environments. In rainforests, there are multiple layers like the canopy, understory, and forest floor, while coral reefs have intricate three-dimensional frameworks built by corals. These structures create countless microhabitats and niches, allowing many species to coexist without directly competing. The relatively stable climates of both ecosystems further encourage species to specialize in narrow ecological roles. I’ve learned that this kind of niche specialization, along with strong biological interactions such as competition, predation, and mutualism, drives coevolution and the emergence of new species over time.

Together, these factors help explain why both tropical rainforests and coral reefs harbor such immense biodiversity. Their complexity, productivity, and stability foster environments where evolution can flourish, leading to the incredible variety of life we see in these ecosystems today.

Personally, I’ve noticed similarities between the two ecosystems in terms of mutualistic interactions and how each species forms a tight connection with others, creating a highly interconnected trophic web. Both systems depend heavily on all components of their food webs, and it was amazing to witness these relationships in action. One key difference I observed was that, in the reef, most organisms relied heavily on coral for both habitat and food. The coral–algae symbiotic relationship was absolutely crucial for the health of the entire ecosystem. In contrast, the rainforest had a broader range of vertical space, with species occupying the ground, understory, and canopy. There also seemed to be more specialized interactions between specific plants and animals, such as the cecropia tree and fungi’s mutualism with ants.

This course was an amazing experience, and I learned so much more than I expected in such a short time. My favorite parts of the course were snorkeling in Glover’s Reef and seeing all the diverse species of wildlife interacting and swimming around. It’s rare to see a reef thriving the way Glover’s was, and that was an unforgettable experience—especially seeing stingrays, sharks, and so many types of fish I knew nothing about before the course. I also loved observing mutualistic relationships in the rainforest. Learning about the ants and the various ways they interact with specific trees and fungi was really cool, especially because some of these examples were straight out of textbooks—seeing them in real life was surreal. My least favorite part? Definitely the bugs! Removing several cockroaches from our room in Las Cuevas and getting eaten alive by sand flies in Glover’s were probably my least favorite moments.

Three things from this course that I know I’ll remember for a long time are the cecropia and ant mutualism, the contrast between MPA and non-MPA reef sites, and seeing glass eels. I was especially fascinated by the cecropia and ant mutualism. It was amazing how two completely different organisms could evolve to rely so closely on each other. I had never seen that kind of relationship up close before, and it changed how I think about the complexity of interspecies interactions. Visiting the marine protected areas and comparing them to non-protected sites was honestly heartbreaking. The difference was so stark—vibrant coral and active fish populations in the MPAs, and lifeless, dying coral in the non-MPAs. It made the consequences of poor environmental management feel very real to me. Finally, one of the most magical moments of the course was seeing the glass eels swimming in the open ocean. I had read about them before, but witnessing these tiny, transparent creatures in person was incredible. It reminded me how much of the ocean’s life cycle is still hidden and delicate—and how much we still have to learn.

Day 8: Adios Las Cuevas!

May 23, 2025

Travel Day! LCRS —> Tropical Education Center (TEC)
We woke up bright and early to clear out of the LCRS. WOW, how the time flew! It really feels like just yesterday we were driving into LCRS for the first time. I was sad to leave, but definitely excited for what was in store! What was that, you ask? A full tour of the Actun Tunichil Muknal (ATM) Cave!
Our tour guide, Rafael, was so great! He told us about how the Maya people used the ATM cave, and then took us into it to show us the ruins. Among them, skeletons from Maya human sacrifice. It was very interesting to hear how much every detail of the cave meant to them, as a representation of the Maya underworld. The placement of their firepits, for example, was designed so that shadows of certain figures resembled their god of rain’s wife, so to them that represented her presence in the cave and her availability to be a conduit for them to pray for rain as well. Overall, this cave was so cool to see and swim through, and we had such a great day.
After that, went to the TEC, settled in, and then went on a night tour of the Belize Zoo. I loved to see all the nocturnal animals, but I also loved to see how the Belize Zoo was dedicated to wildlife conservation. The only animals they had were kept from the wild either because they were orphaned and raised by humans (and therefore could not survive on their own in the wild) or if they were medically unable to return to the wild, like the Jaguar Martin, who lost his eye due to glaucoma. And, as always, it would not be a Sam blog post without an ant mention! Back at the TEC, I saw a cute “Leafcutter Ant Crossing” sign that was displayed over one of the leafcutter’s highways. I loved it!
Tomorrow, I’m looking forward to an awesome boat ride to the beautiful Glover’s Reef Research Station (GRRS) 80 miles off the coast of Belize City in the Caribbean Sea!
<3,
Sam
Images
ATM Cave Welcome Sign
ATM Cave Skeleton (formerly known as Crystal Maiden, no longer certain of gender so this name is out of style) (https://www.atlasobscura.com/places/the-crystal-maiden-of-the-actun-tunichil-muknal-cave-belize)
Belize Zoo Sign  (picture from Lily Hestjean)
Jaguar at Belize Zoo (bad picture, sorry!)
Leafcutter ant crossing sign

The Ant-Man Super Bowl: Leafcutter Nest Excavation

May 20, 2025
Hello again! As you can tell from the title, today was yet another amazing day at Las Cuevas Research Station. The day began with some light birdwatching, during which we saw some flying macaws, a toucan, and a Northern Mealy Amazon parrot. These were very cool to see!
Our morning and half of the afternoon was filled with finishing up the Cecropia experiment and listening to presentations. Before I get into what we did with the second part of the afternoon, I’ll illustrate the results of our experiment. As you can see from the poster below, the question “was how do young cecropia trees defend themselves if they’re not colonized and protected by a colony of Azteca ants?” Unfortunately, our results were inconclusive. Due to limitations on time, we weren’t able to catch Orthoptera herbivores of the same species, so that factor was not standard, and we also had the problem of hydration of the leaves (we can’t know if the leaves were absorbing water, or how much they had at the time oof weighing, because we are in a field station with only one afternoon and one morning to devote to this project.) So, the results were inconclusive, but you can read our poster pictured below. (The poster was a collaborative effort; if you know me, you know that’s not my handwriting.) After the project was over, we had a presentation from the Rafael Manzanero, director of the Friends for Conservation and Development, which is a really cool Belizean NGO that manages the LCRS, Chiquibul National Park and Forest Reserve, and they defend it. They have armed rangers, filling the role that the government plays in the US. It was very interesting to hear from him. After that, I gave my Ants Taxon Briefing, Lily gave hers on Epiphytes, and Dyllan talked about plant-insect interactions. During those presentation, we also heard from Yasmini Manzanero, the FCD’s Cultural Heritage and Karst Expert, and she briefed us on the cave system we are to explore tomorrow.
After those presentations, we had the ant-man Super Bowl: we excavated leafcutter ant nests to find their fungal garden. This was truly exciting. First, we approached the young colony (~1 year in age, 1 entrance) which is in the clearing right next to Jane’s (LCRS Manager) cabin. This is an optimal location for a Leafcutter colony because they like forest edges. The edges provide easy access to fresh vegetation for their fungus, while also providing the queen easy access to get into the ground quickly. We began our excavation by digging next to the colony, because fungal gardens (which is where the queen is) aren’t usually underneath the colony entrance, but they’re off to the side. Excavating this way minimizes damage to the colony’s structure and garden. This was so fun, because Dr. Solomon pulled out the entire fungal garden on a large kitchen spoon. With the whole garden came the queen, which was also awesome to see. She was so huge, because she has to store her sperm for up to 20-25 years and she also has to store her fungus when she leaves the colony she’s from, so she had a lot going on. It was super cool to see her.
Once we had thoroughly explored the young colony, we approached the mature colony. The mature colony was ~x years old and had many entrances. We selected this mature colony because it was raised, meaning the fungal gardens would be easier to access. This colony presented a problem though: the many hundreds and thousands of soldier ants that swarmed as we excavated. This meant that everyone had to put their things far away, and continually stomp soldier ants off their boots so they didn’t chew through them. As the ant-man, I was excited to see the horde of ants flowing out of the nest like a mighty stream and I grabbed the shovel. I was mostly successful, but I did get one battle wound. This was quite a different wound, as it hurt in the moment but it faded quickly. It also bled a lot, which was concerning (and turned my stomach bc I do not like blood very much) but that also subsided quickly. All in all, it was a great ant-super bowl and I’m super proud to have been a part of the excavating. After the wounds, we found the fungus. This fungus was interesting because a) there was much more of it, naturally, and b) It had a different texture than the fungus of the young colony. I’d be interested to compare them in a lab.
Following dinner, we had our first night hike. We saw a rare Pauroque bird (one of the “heard not seen” birds,) but the coolest thing we saw was the leafcutter ant highway. During the day, you see a couple or even a steady stream of ants flowing on these highways. At night, this highway was PACKED. It probably looked like the Katy Freeway when they brought 45, 90, and 180 to Rice. It is pictured below.
Images:
Cecropia Experiment Poster (if you have seen me write, you know that I did not write this. It was a collaborative effort.)
Battle wound from Excavation of Mature Colony (Got bit by Soldier Atta cephalotes)
Young Colony’s Fungal Garden
Ant Highway During Night Hike

Parrots, Ants, Beetles oh my!

Hi! Day 3/5 in the jungle! Today we listened to a lot of presentations. One was by the head of FRC where we got to learn more about their mission and role they play in LCRS and the surrounding areas. The other was about the caves here in LCRS and the cultural importance of them.

Then we did a leaf cutter nest extraction. We found  young leaf cutter ant that looked to be about 1.5 years old and had few ants. We got to see the queen on the fungal garden and see how big she was! This nest was young enough that the hive had no army ants yet. We then extracted an older leaf cutter nest that was about 20-25 years old! That one definitely had army ants. We were stomping our boots left and right. This nest had a guest cockroach and was absolutely massive. We dug in through the side to find the fungal garden of that nest as well. That fungal garden was huge with multiple chambers.

We then went on a night hike to finish the day. We put on our head lamps and headed out. Unfortunately we didn’t see much but a few cockroaches, tarantulas and other insects. We did see a lot of beetles though! There was one during dinner and a few during the various hikes throughout the day. The beetles we found were dung beetles and herbivores beetles.

Day 4 (LCRS Day 2): Pee, Cecropia Trees, and even more ants!

May 19, 2025
Hey hey hey! Today was, like yesterday and the day before, a fantastic day. We worked on two projects today, the first being a pitfall trap experiment to assess nitrogen limitation in arthropods in the canopies versus the forest (underlying assumption: canopies have less nitrogen. Hypothesis: arthropods of the canopies will exhibit more signs of nitrogen limitation (be more attracted to nitrogen-rich fluid (human pee) than arthropods of the forest floor.) In the afternoon, we began an experiment with Cecropia trees. This was exciting for me, because I am the ant man. These trees, like the Bull’s Horn Acacia mentioned earlier this week, are protected by a colony of Azteca family ants living inside them (except Bull’s Horns have Pseudomyrmex ants in them.) The experiment considered whether young Cecropias found other ways to protect themselves from herbivores before they were inhabited by ants, so the experiment didn’t directly involve ants, but that didn’t stop us from studying how the colony moves about the tree.
The first tree we found was inhabited by a whole colony, and it was so cool to see them swarm to protect the tree and to see the tree’s structure to provide a home for them. The second tree we found, after a long search, was much younger and had only one inhabitant: the queen. She was laying larvae, and the tree was not yet colonized, so we sued it for our experiment and called it day there.
However, throughout the day, we saw many cool ants. In the morning, doing the nitrogen-limitation pee experiment (where we peed in tubes to see if arthropods would be more attracted to our nitrogen-rich pee compared to water,) we saw a leafcutter ant colony that must have been 30-40 feet long across the trail. SUPER cool. Right next to the colony (or right above,) there was a Bull’s Horn Acacia Tree coexisting with the Leafcutter colony. We decided to run a fun experiment by putting a leafcutter soldier on the acacia tree to see what would happen. Unfortunately, while attempting to rouse the ants who lived on the tree, the leafcutter soldier fell off so the experiment was cut short. We got to see it run away from one of the Pseudomyrmex guard ants though, which was pretty cool.

In the afternoon, when looking for a cecropia tree, we naturally ran into the cecropia ant inhabitants. Our afternoon experiment will test whether or not young (not colonized by ants) cecropia trees will develop alternate (chemical, physical) defenses to herbivorous predators before they are colonized by ants and benefit from that defense. When we happened upon an adult cecropia tree, we got to see how they swarm out when it falls, and how the tree has evolved a hollow structure inside of it for the ants to live. The structure is an akin to the ants having high-rise apartments, because that tree is tall! Cecropia ants all fall under the Azteca family, and I think we saw Azteca alfari based on the looks. After we saw the big cecropia tree & colony, we set out to find the young uncolonized tree. While we searched, we happened upon two more really cool ant phenomena. The first, we saw a young leafcutter ant colony. We could tell it was young for two reasons: a) the colony entrances were small. there were multiple, indicating an age greater than one year, but still relatively small and therefore young. b) the ants were bicolored (red head and abdomen and a darker thorax.) Dr. Solomon did some inconclusive research as to why leafcutter ants may be discolored, but he discovered that ants were bicolored when the colony was young, which is how I know that colony is young! After the young colony, we saw an ant I hadn’t seen yet or even researched: theCamponotus sericeiventris, or Carpenter ant (but it directly translates to “Golden butt” ant, which is more fitting.) After we saw these two ants, we found the young cecropia tree, which was supposed to be uncolonized, but the one we found had one singular inhabitant: the queen! It was super cool to see her working in the little tree before her colony and her tree grew (her colony and tree did not grow because we cut it down unfortunately.) Overall, it was another fun and interesting day to be the ant man, and I know tomorrow will be even better as we are to spend the afternoon investigating leafcutter ant colonies! What a treat!

Ttylxox,
Sam
Images:
Me with my Pee tubes for the nitrogen limitation experiment:
Huge leafcutter ant colony
Bull’s Horn Acacia directly next to/ on top of huge leafcutter colony
Colonized Cecropia tree filleted open
Bicolored leafcutter ant (Image from https://leafcuttingants.com/forum/viewtopic.php?t=531&start=10)
Golden butt carpenter ant (Image taken by Dr. Solomon) (I know it doesn’t look gold, the gold part has mostly fallen off)
Young Cecropia tree filleted open showing only the queen inhabitant

Day 4: Doing P Tests (kinda)

Day 4 started by being asked to find the differences in nitrogen limitations on the forest floor and in the canopy. We wanted to make a trap that insects could be attracted to and fall and get stuck in. That means we needed some nitrogenous liquid. One might think finding this is simple, and it was, BUT it’s not pretty. We used our pee in vials, hung them on trees and dug the vials into the ground, and are now waiting to see what is attracted in each location. While it is not a glamorous lab, the look on everyone’s faces when we were told to pee in vials that would be used in the project was hilarious. 

  

(Nitrogen experiment setup diagram)

(Swinging like Tarzan on a water vine!)

After lunch, we set up a second experiment where we tested how the colonization of the cecropia tree with ants vs an uncolonized young cecropia tree varies in defense mechanisms (chemical and physical). Cecropia trees and ants have a mutualistic relationship where ants form their colony in this tree in exchange for protecting the tree from herbivores. We wanted to know if leaves would potentially be thicker, gross, or poisonous before having the ants  colonize the tree. We tested this by collecting herbivorous insects in boxes and adding leaves from colonized and uncolonized trees to see which got eaten more. 

(Inside of colonized cecropia tree; looks like a ant high rise apartment building)

While hiking to collect the insects and leaves, I saw a large brown skink (lizard) that I have not identified yet. It was about 8 inches and had a long tail. I also saw a silky anole while hiking for setting up the nitrogen lab. Both were on the side of the trail in the shade. 

The last two exciting notes about today are potentially the most exciting. First, I got a great look at a toucan in the tree right out front of Las Cuevas! It was not too close, but I used my binoculars and saw the red tail and the large yellow beak very clearly. 

Additionally, I heard something loud making screaming sounds from inside the jungle after it got dark. I have been made aware that one animal is known for making a screaming noise, and that is the puma. Hopefully our camera traps catch a picture of it walking by! 

See yall soon!

Claire C

Toucans, Pee Tubes, and Ants – A Day in the Forest

Today’s bird highlight: a toucan! It didn’t stick around for long—just a quick flash of color as it flew off—but still exciting to catch a glimpse of one in the wild. My taxonomic group is birds, so I’m always keeping an eye (and ear) out for them. I’m really hoping to get more chances to go birdwatching soon and see a wider variety of species. Just catching that brief glimpse of the toucan made me want more—I’m especially curious to spot some hummingbirds or maybe a trogon next time.

We tackled two different projects today. The first one focused on how nitrogen availability might affect invertebrates living in the canopy compared to those on the forest floor. To test this, we set up tubes filled with either water or nitrogen (yes, our pee!) both in trees and in the soil, with each station spaced 50 feet apart. Hopefully, this will help us understand if nutrient availability plays a bigger role higher up in the forest.

The second project explored how Cecropia trees protect themselves. In their early stages, they don’t have ants living in them yet, so we’re curious to see how their defenses differ before and after they recruit their ant allies. It’s a fascinating example of mutualism in action.

Fieldwork is full of surprises—some feathered, some scientific. I’m looking forward to the next chance to get out early, binoculars in hand, and see what’s flying through the canopy.

Day 4: Into the Thick of It

IMG_0329Hi! We’re still in the heart of the jungle, on day 2/5 at Las Cuevas. Today was a busy day with us successfully setting up two research projects!

This morning we set up a research experiment using pitfall trap to try to see the nitrogen limitation between the forest canopy and ground by seeing the difference between invertebrates in the nitrogen (our pee surprise!) and the water pitfall traps. We spent the morning setting up the experiment on several trees.

In the afternoon we set up another research experiment to see if bullhorn acacia trees change their defense mechanism based on age and colonization of ants. Usually a Bullhorn Acacia tree has a mutualistic relationship with ants where the ants live inside the hollow tree and are provided food by the tree. In return the ants provide a protection to the tree from herbivores. We collected leaf samples from a young and old acacia tree and several different species of invertebrates from grasshoppers to crickets to katydids and put them inside with the leaves to measure change in biomass and monitor the insects health with close proximity to the plant.

Along our treks on the woods we observed several different cool species from a queen ant who had just mated her larvae in a young acacia tree to many different tarantulas. We also saw several beetles. Of note Sam found a Tiger Beetle which is really cool because those beetles are very fast!

LCRS Full Day 1 and First Big Project! (And, as always, ants!)

Day 1 at Las Cuevas (May 18)
Today was another great day here at Las Cuevas! We began our first big project for the class, and the prompt was to use motion-detection cameras in some way. We decided it would be best to use them to assess animal motion on man-made trails (disturbed area) versus undisturbed area. We settled on the research question: How does the presence of man-made trails influence the biodiversity of the vertebrate animals in the immediate vicinity surrounding the LCRS (Las Cuevas Research Station?)
We decided to set up cameras both on trails of all sizes (Small Mayan and Bird Tower Shortcut Trails, Intermediate Monkey Tail Trail, and along the overgrown San Pastore Road and the more maintained main access road) and for each camera set up on a trail, there was one ~50 steps into the woods to monitor undisturbed animal activity. My camera was set up in the woods near the Monkey Tail Trail, at N 16.73019, W 088.98109, in case you were interested.
It wouldn’t be my blog without this next part: the ants of today! The morning was fairly ant-less. There were ants, but I didn’t see anything too noteworthy. The afternoon, though, was quite fun. Early on (first 5 meters of the Bird Tower Shortcut Trail) we saw a leaf cutter ant pathway breaking up leaf litter. I thought it was cool how the leaf litter was broken up in their path, that shows often they walked it & how many there were. After that, we saw a leaf that had been cut up by a leaf cutter ant, which was really cool! Dr. Solomon shared that these ants have zinc-lined (really really hard) mandibles with which they clamp on to the edge of a leaf, vibrate (like a jigsaw,) and rotate their bodies to carve out a semi-circle shaped piece of leaf roughly the size of their bodies. In the image below, the size of the semi-circle gives the size of the ant! After a bit of time, I was bored while people were setting up their cameras, so I went and caught a leaf cutter ant with the pair of forceps (pictured below!) This was the first of what I assume will be many ants caught this week. After that, I found a Pseudomyrmex family ant on a flower. I was initially puzzled because this was a less likely species to see, but when I realized the large silvery eyes, I could tell it was a Pseudomyrmex. If I had to guess specific species, I would guess Pseudomyrmex gracilis. That’s all I got ant-wise for today.
What a great day, and I can’t wait for the adventure tomorrow will bring!
Images:
Defined ant path in leaf litter
Leaf cut up by Leafcutter ants
Me holding the leafcutter ant I grabbed with forceps
Image of Pseudomyrmex ant found on flower. Apologies for poor quality.