Tag Archives: mutualism

Parrots, Ants, Beetles oh my!

Hi! Day 3/5 in the jungle! Today we listened to a lot of presentations. One was by the head of FRC where we got to learn more about their mission and role they play in LCRS and the surrounding areas. The other was about the caves here in LCRS and the cultural importance of them.

Then we did a leaf cutter nest extraction. We found  young leaf cutter ant that looked to be about 1.5 years old and had few ants. We got to see the queen on the fungal garden and see how big she was! This nest was young enough that the hive had no army ants yet. We then extracted an older leaf cutter nest that was about 20-25 years old! That one definitely had army ants. We were stomping our boots left and right. This nest had a guest cockroach and was absolutely massive. We dug in through the side to find the fungal garden of that nest as well. That fungal garden was huge with multiple chambers.

We then went on a night hike to finish the day. We put on our head lamps and headed out. Unfortunately we didn’t see much but a few cockroaches, tarantulas and other insects. We did see a lot of beetles though! There was one during dinner and a few during the various hikes throughout the day. The beetles we found were dung beetles and herbivores beetles.

Belize Day 4: Buggin Around

This morning, Elise and I discovered that there was definitely maybe a jaguar (or some other feline-y mammal) strolling around our cabin. Last night, when we were frolicking around the clearing of grass around our cabin, trying to spot stars whenever the stubborn clouds parted, we smelled a bold whiff of something like cat pee. As soon as our nostrils made us suspicious, we fell silent. Then, we heard a few twigs snap and RACED back up the stairs to our safe cabin deck, saving the jaguar spotting for another night. Debriefing with Dr. Evans this morning, we learned that he also heard a deeep mammal exhale right outside of his room around that time. 

With coffee and tea in hand, sitting around the picnic tables on the deck, we listened to our reliable grackle friend (he’s there all day, every day) squawk and put on a scene for the ladies. Down on the grass, Lonesome George (a flamboyant, wild turkey, who doesn’t mind being alone so that all eyes are on him) was roaming around. He’s extra flashy with an undercoat of black and white checkered feathers, some iridescence on his top coat, and quite a few more patterns. Also, we found a bunch of turkey feathers in a spot along the trail and think a jaguar got to his friend. 

(Lonesome George – 05/20/25)

Today we would be designing and executing another research project, this time, using our pee for an experimental treatment. Often, most of the rainforest’s nitrogen is stored near the forest floor in the decomposing leaf litter and nitrogen-fixing bacteria (organisms that live in the soil and convert atmospheric nitrogen into forms usable by plants). As you climb higher, nitrogen becomes harder to come by. Therefore, plant-available nitrogen should be more available and more evenly distributed on the forest floor than in the canopy, where occasional, limited nitrogen sources would be more concentrated around the few sources and less common. To test this, we set up pitfall traps, which were just tubes filled halfway with liquid, camouflaged so that insects would stumble in and stay trapped. We would compare the differences in species richness (the number of species), species abundance (the total number of species), and total biomass in the treatments. To make the control and experimental treatment, we filled tubes with water (no nitrogen) and pee (yes nitrogen) hidden in the forest floor (in the soil) and on tree trunks. We peed in tubes, put on our boots, and headed out for the rainforest.

(Tree Pee Tubes – 5/19/25)

Before we headed out, we were given 2 pieces of advice for the field:

  1. Dr. Evans: “Make sure you guys bring your childlike whimsy with you.” 
  2. Dr. Solomon: “Watch out for killer ants.”

About the killer ants, apparently not too long ago (around the 90s), scientists decided to breed the European honey bees (with low honey production and low agressiveness) with African Honey Bees (with high honey production and high agressiveness) with the hopes of creating a high honey and low agressiveness phenotypic combination and a winning bee vareity for market honey production. Instead, they created the opposite–a low-honey, high-aggressiveness bee. Not only is this meany more aggressive, but it also has a lower threshold (it will react with less disturbance/irritation) for sending a warning call back to the hive to bring the other bees over. So, contact with one hybrid bee could summon the killer bees. 6 of these hybrids (specifically queen bees) escaped the lab, and now, 30ish years later, they’re taking over the rainforests. 

While setting our traps, we stopped to chat about some more insects that can do some damage. We walked on top of this humongous leaf-cutter ant hill (literally hill-sized), and some of the pinky-sized, pinch-armed soldier ants (the specialized protectors of the colony) came out to greet us. One of us turned around and saw a soldier making its way up Ian’s back. While everyone was panicking and working together to whack it off, Dr. Solomon popped by and said, “So guys, that was a great example of social grouping in primates. All you have to do now is eat the ant.” Yep, classic Dr. Solomon. Learning by doing, ya know?

(Leaf-cutter Soldier – 05/19/25)

After this little morning project and a delicious lunch from our exceptional chef Angie (nothing can compare to the plantains here), we strategized for rainforest study 3, which did not involve urine, but rather catching crickets and ravaging ant colonies :(. There are these trees called Cecropia trees, which are layered with chambers like a “high-rise apartment complex” that mutually host Azteca ants. The ants protect the tree from predators, and the tree excretes sugary, nutritious meals for the ants in return. 

DSCF1315 (Cecropia-ant Mutualism – 05/19/25)

In our project, we were examining how the presence of the ants changes the tree’s physical and chemical composition (specifically its defense systems) in its leaves. We would collect leaves from two Cecropia trees–one with ant symbionts and one without–and compare the strength and herbivory amounts of the leaves. We had a fancy penetrameter to measure the force it would take to break through a leaf, and Dyllan brought out her butterfly net so that we could catch some herbivorous insects to consume the leaves. 

You know us, and we stopped many times during the search for Cecropia trees to poke sticks in freshly webbed-covered tarantula holes. When finally one popped out, the whole class exclaimed “OH!” and jumped back in unison. Also, did you know spider hairs are also irritating in addition to their venomous fangs? Their hairs are barbed and urticating, meaning they cause irritation and discomfort upon contact (https://enviroliteracy.org/animals/are-tarantulas-hair-poisonous/). 

Today was a bug-heavy day, completed with a scorpion sighting in our classroom drawer. My takeaways: insects are very diverse, have a few defense tricks up their sleeves (their 6 or 8 sleeves), and are mind-boggingly complex. Tomorrow, we’re learning more about leaf-cutter ants, so stay tuned for more insect lore. 

  • Lily 🙂

Day 4 (LCRS Day 2): Pee, Cecropia Trees, and even more ants!

May 19, 2025
Hey hey hey! Today was, like yesterday and the day before, a fantastic day. We worked on two projects today, the first being a pitfall trap experiment to assess nitrogen limitation in arthropods in the canopies versus the forest (underlying assumption: canopies have less nitrogen. Hypothesis: arthropods of the canopies will exhibit more signs of nitrogen limitation (be more attracted to nitrogen-rich fluid (human pee) than arthropods of the forest floor.) In the afternoon, we began an experiment with Cecropia trees. This was exciting for me, because I am the ant man. These trees, like the Bull’s Horn Acacia mentioned earlier this week, are protected by a colony of Azteca family ants living inside them (except Bull’s Horns have Pseudomyrmex ants in them.) The experiment considered whether young Cecropias found other ways to protect themselves from herbivores before they were inhabited by ants, so the experiment didn’t directly involve ants, but that didn’t stop us from studying how the colony moves about the tree.
The first tree we found was inhabited by a whole colony, and it was so cool to see them swarm to protect the tree and to see the tree’s structure to provide a home for them. The second tree we found, after a long search, was much younger and had only one inhabitant: the queen. She was laying larvae, and the tree was not yet colonized, so we sued it for our experiment and called it day there.
However, throughout the day, we saw many cool ants. In the morning, doing the nitrogen-limitation pee experiment (where we peed in tubes to see if arthropods would be more attracted to our nitrogen-rich pee compared to water,) we saw a leafcutter ant colony that must have been 30-40 feet long across the trail. SUPER cool. Right next to the colony (or right above,) there was a Bull’s Horn Acacia Tree coexisting with the Leafcutter colony. We decided to run a fun experiment by putting a leafcutter soldier on the acacia tree to see what would happen. Unfortunately, while attempting to rouse the ants who lived on the tree, the leafcutter soldier fell off so the experiment was cut short. We got to see it run away from one of the Pseudomyrmex guard ants though, which was pretty cool.

In the afternoon, when looking for a cecropia tree, we naturally ran into the cecropia ant inhabitants. Our afternoon experiment will test whether or not young (not colonized by ants) cecropia trees will develop alternate (chemical, physical) defenses to herbivorous predators before they are colonized by ants and benefit from that defense. When we happened upon an adult cecropia tree, we got to see how they swarm out when it falls, and how the tree has evolved a hollow structure inside of it for the ants to live. The structure is an akin to the ants having high-rise apartments, because that tree is tall! Cecropia ants all fall under the Azteca family, and I think we saw Azteca alfari based on the looks. After we saw the big cecropia tree & colony, we set out to find the young uncolonized tree. While we searched, we happened upon two more really cool ant phenomena. The first, we saw a young leafcutter ant colony. We could tell it was young for two reasons: a) the colony entrances were small. there were multiple, indicating an age greater than one year, but still relatively small and therefore young. b) the ants were bicolored (red head and abdomen and a darker thorax.) Dr. Solomon did some inconclusive research as to why leafcutter ants may be discolored, but he discovered that ants were bicolored when the colony was young, which is how I know that colony is young! After the young colony, we saw an ant I hadn’t seen yet or even researched: theCamponotus sericeiventris, or Carpenter ant (but it directly translates to “Golden butt” ant, which is more fitting.) After we saw these two ants, we found the young cecropia tree, which was supposed to be uncolonized, but the one we found had one singular inhabitant: the queen! It was super cool to see her working in the little tree before her colony and her tree grew (her colony and tree did not grow because we cut it down unfortunately.) Overall, it was another fun and interesting day to be the ant man, and I know tomorrow will be even better as we are to spend the afternoon investigating leafcutter ant colonies! What a treat!

Ttylxox,
Sam
Images:
Me with my Pee tubes for the nitrogen limitation experiment:
Huge leafcutter ant colony
Bull’s Horn Acacia directly next to/ on top of huge leafcutter colony
Colonized Cecropia tree filleted open
Bicolored leafcutter ant (Image from https://leafcuttingants.com/forum/viewtopic.php?t=531&start=10)
Golden butt carpenter ant (Image taken by Dr. Solomon) (I know it doesn’t look gold, the gold part has mostly fallen off)
Young Cecropia tree filleted open showing only the queen inhabitant

mutualisms and morphos

It seems fitting that my topic presentation is tomorrow because today we saw so many great example of plant insect interactions that I plan to discuss in my presentation! 

To start of the day, we set up pitfall traps around the forest to collect insects. We made one set of traps using our own pee as a source of Nitrogen to attract the bugs that are nutrient deficient. When Dr. Solomon first said we had to use our pee, I totally thought it was a joke, but no. We did in fact hide vials of our pee on trees and in the ground to observe how forest structure impacts nutrient limitation. 

On this hike, I saw another blue morpho, which I was able to get a (blurry) picture of. In real life, the butterfly is big, metallic, and majestic, but in the photo is more of a blue blur. 

Another cool thing we saw was lichen  growing with visible sporophytes. These little adorable contraptions allow the lichen to reproduce by releasing spores for the gametophytes. I’ve read about this process, but it was so cool to actually see it!

After lunch, we went out on a hunt for a colonized and uncolonized cecropia tree, as well as 6 herbivore insect generalist for our next project. Me, Serenity, and Claire immediately caught to katydids in the station and then we set out to find the rest.

We found our first cecropia tree pretty quickly, and we cut it down. Immediately, the ants that live inside of it began to swarm, defending they home and source of food. This symbiotic relationship is covered in my presentation tomorrow, so it will be a cool call back. Additionally, next to this tree, was an Acacia tree that also started swarming with ants once we disturbed it. This is the first of a few Acacia trees that we have seen, which is so exciting because this is another classic relationship that I will cover in upcoming presentation. As we searched for the uncolonized tree, we found a bug fig tree, where we discussed the fig tree and fig wasp relationship, where the wasp eggs are placed in the fig and develop within the fig— yet ANOTHER classic relationship that I will talk about tomorrow. Studying these relationships up close is so cool after learning about them for so long. 

Along our walk, we made many pitstops to try and catch some flighty insects. This was so fun for me. At this point, the weather was much nicer and we were essentially just wandering around trying to catch some useful bugs. We were trying to find two individuals in the same species of orthoptera, but instead we found and caught 4 different species of grasshoppers and one cricket. While this wasn’t what we wanted, it ended up working and it was a blast running around trying to catch them. I also caught a bunch of nymphs and adults of this red insect that we keep seeing hundreds of on all of our paths. I thought at first we could use them in our experiment, but I soon saw that they have haustellate mouths instead of mandibulate, which would be more ideal for our experiment. While we couldn’t definitively ID them, We think they were true bugs. I kept collecting them because I thought they were cool. I thought they were even cooler when they started fighting hunger games style, with the larger ones sucking the bug juices of the smaller ones. We kept them in a jar to see who is the final winner tomorrow. 

In addition to these bugs, I saw another Dirce beauty on the road, and I got a good picture and came so close to catching it! I don’t think butterfly catching is particularly my strong suite, but I still have hope. Maybe tomorrow will be the day.

Day 6: we did not want to take any risks

Today’s general agenda: project leaf diversity —> project mutualism —> bird tower 

Having given my presentation on ants, I was beyond excited that we were going to focus on ants even more on this trip! In particular, today, we were tasked to examine the relationship between Azteca ants and Cecropia trees. These two different species form a mutualistic relationship where both parties benefit by working together. The ants get food while the tree gets protection. We wanted to know how Cecropia trees, before hosting these ants, protect themselves from being eaten. 

click for video showing the Azteca ants and Cecropia trees

720p

We have been constantly examining organisms on the ground that we decided to change up our perspective this evening. Near the research station is a bird tower that is three stories high. From the bird tower, we got to observe the Chiquibul forest in its entirety. We also got to see the Belize sunset. I don’t know if it was the wind, the greenery, or sunset, but, in the moment, I never wanted to get off the tower. Of course, all good things have to come to an end, so we turned on our headlamps and hiked back. 

selfie on the bird tower!

As I was carefully walking in the front, I hear Pierce call out “snake!” in the back. The snake turned out to be a venomous jumping viper hidden in the leaf litter! Though the snake was small in length, we did not want to take any risks. We kept our distances and safely moved forward.

Each night, as you may know, we give presentations that focuses on either specific taxonomic groups or topics. Today, Liz addressed tropical diseases. I was excited to learn more about Dengue Fever because the disease is somewhat prevalent in Taiwan. We even talked about how climate change can affect Dengue Fever. In short, climate change can exacerbate Dengue Fever and increase its range. 

Brendan Wong

Las Cuevas, Belize

5/19/2019