Tag Archives: pee

June 19th: Rainforest Experiment Results!

Today we concluded both our pee pitfall experiment and the camera trap experiment! This morning we made a short hike through the Maya trail, and collected each of our pitfall traps filled with urine and unlucky arthropods. Along the way I spotted some cool looking harvestmen climbing along a sharp give-and-take palm! We also saw some really cool examples of moths that were killed by the “zombie” fungus Ophiocordyceps which takes control of the nervous system of its victims. These moths were in various stages of decay so it was like we were seeing the process itself, which was super cool!

 

Once back in the lab, we sorted our pee pitfalls and removed all of our captured arthropods to tally which traps had the most visitors. We found that in general, the arthropods were more often found in the nutrient rich pee, and there were many more arthropods found on the forest floor than the canopy. But the canopy arthropods actually had more guests in the water tubes, but the sample size for this area was very small. I actually did fish out a few harvestmen and a small spider during our data collection, so I did get some arachnids in there! Check out our title for the poster aka our most despicable pun yet (Urine for a Treet).

Some of the students went out to go collect all of the camera traps we had placed on our first day in the rainforest, and during this spotted a really cool spider, I wish I had been there! I have tried my hardest to identify this fuzzy guy just from the awesome picture Elena sent me, but still no luck. Its leg morphology points to it being some kind of ground-dwelling spider rather than orb-weaving. The coloration, leg shape, and fang placement resembles that of both huntsman and wolf spiders, but none of the spiders I’ve researched in this area have such distinctive fuzzy legs. What a cool little mystery! Fun fact, the “hair” on spiders legs are actually called setae and can be used for grip when moving around, sensing chemicals, and so many other cool things!

Pee Experiment, Leafcutter Ant Excavation, and Night Hike! 

After hearing a lovely morning lecture from Michael on the importance of tropical soils and their nutrients, we designed a classic TFB experiment: pee tubes. We wanted to see whether arthropods in the canopy or the forest floor are more attracted to the nitrogen in our urine and what that might say about the scarcity of nutrients in these areas. We all peed in tubes, compared hydration levels for fun, and then set out to lay these pitfall traps in the forest. During this I actually spotted a jumping viper on the trail right before we were about to step on it which was a little terrifying, but it never struck. Someone also spotted the adorable and rare eyelash viper curled up on a tree! 

BIOS 319 pee gradient

After that adventure we had our very own leafcutter ant expert Dr. Solomon show us the inner workings of these colonies (including their fungal gardens!), which was so fascinating to see! During our search for a suitable candidate, we found so many arachnids! Dr. Correa found a bunch of tiny scorpions under a damp piece of wood, as well as a dead tarantula. We were also able to spot two golden silk orb weaver spiders in their web which was really cool! But the star of the show was definitely the Mexican burrowing toad we found while digging around! He was just such a fun looking guy he was so gelatinous, had no neck, and the most powerful little back legs. He was fantastic

Golden orb-weaver spider
Qur amphibian expert Rusty holding the beloved toad

Then to top off a fantastic day we had an eventful night hike that definitely broke some TFB records! We took a short journey to a pond nearby and along the way saw TWO Morelet’s Tree Frog (which are a rare endangered species) both of which jumped onto some students and hung out there while we tried to photograph it which was so cool! We had to be very careful not to let it touch us if we had sprayed bug spray because they can take in chemicals through their skin, but they were so friendly! And then we saw the insane, documentary worthy event of a snake winding up a tree and eating sticky tree frog eggs! There were snakes everywhere today. It was so insanely cool! I wonder how many more cool species we can find! 

D-11 The queen and the toad

Hello everyone!

Today, during the first half of the day, we went out for a second experiment at the rainforest. We were first told that for this experiment we would use pee. Hearing this I began to mentally prepare myself to literally pee in the rainforest, thankfully our instructors had other plans in mind. Their plan was much more simple, it involved us taking a small flask and peeing into it in the restroom, then planting it in the floor of the rainforest, and placing another flask of pee on the trunk of the tree. By doing this we would be able to estimate the biomass of arthropods in the Chiquibul (at least in the trees we sample!) The use of pee would help us see in what region, whether trees or the ground, arthropods sought out nutrient rich material. This time around our trekking was made really interesting by several sightings! We saw two snakes, a jumping pit viper and an eyelash viper. After placing our containers, we went back to LCRS to get lunch, which was delicious!

After lunch we all headed out again, yet this time our aim was very different, we were all seeking leaf-cutter ant hills! We eventually found one that was about a year old. Using a small shovel we reached the inner chamber of the nest where the fungi is harvested. We even found the queen!

Then we went to inspect a much larger ant hill, yet no ants came out, instead we found a Mexican burrowing toad!

We released the toad and continued our search for a hill!

At the first ant hill I saw one of my taxa, a western honey bee. It became attracted to my backpack and begun hovering around it. I’m not completely sure what attracted it, but I have a theory that it was the Gatorade in one of my bottles. I didn’t get a good photo of the honey bee, yet throughout the day I saw a few others that I was able to photograph! For example, here is a bumblebee that I saw!

And here is a photo of a type of stingless bee not found on my taxon sheet!